• Sums of 1-parameter functions, such as convoluted exponentials in fast decay processes;
• Optional global analysis of up to about 50 data sets with the same decay constants (e.g., multi-wavelength sets);
• Optional robust correction for response function in fast fluorescence decay using standards [1].
• Running on a wide variety of computers.

Methods:

• fully automatic: no starting estimates needed for the number of components or for their parameters;
• modified Gauss-Newton least squares, with intensive searches from many starting points to find the global optimum;
• optional spline approximation of model functions [2,3] for faster analyses (probably unnecessary with current computers).

References:

1. R.W. Wijnaendts van Resandt, R.H. Vogel & S.W. Provencher: Double beam fluorescence spectrometer with subnanosecond resolution: Application to aqueous tryptophan. Rev. Sci. Instr. 53, 1392 (1982).
2. R.H. Vogel: SplMod User's Manual (1988).
3. S.W. Provencher & R.H. Vogel: Regularization techniques for inverse problems in molecular biology in: Numerical Treatment of Inverse Problems in Differential and Integral Equations, eds. P. Deuflhard & E. Hairer (Birkhäuser, Boston, 1983), pp. 304-319.

Downloading:

1. README.txt (2.0KB): Short guide for the installation and test run; read this first;
2. splmod-manual.pdf (3710 kB);
3. hd83.pdf (1237 kB) (useful, but not necessary reading);
4. splmod.for ( 72KB): (zipped with gzip) Fortran source code;
5. splmod.in (2.7KB): Test data.